TCID₅₀ Assay

Overview
A gold-standard assay to determine the dilution of a virus that infects 50% of cell cultures, widely used for virology research and vaccine potency evaluation.

Principle

• Serial dilutions of virus are added to cells in 96-well plates.

• After incubation, cytopathic effects (CPE) are observed.

• Viral titer is calculated using the Reed–Muench method.

Applications

• Standardizing viral inoculum for experiments

• Vaccine potency and challenge studies

• Antiviral drug efficacy evaluation

Deliverables from MIV
✔️ Viral titers reported in TCID₅₀/mL
✔️ Representative well images of CPE vs. control
✔️ Full Reed–Muench calculation sheets
✔️ Expert consultation for interpretation