Neutralization Assay

Overview
Measures the ability of antibodies to block viral infection, serving as the gold standard for evaluating functional immunity.

Principle

• Serum samples are mixed with live or pseudoviruses.

• Mixtures are added to susceptible cells.

• Reduction in infectivity is quantified (IC₅₀ or PRNT₅₀).

Applications

• Vaccine efficacy testing

• Clinical immunity assessments

• Antibody therapeutic evaluation

Deliverables from MIV
✔️ Neutralization titers reported as IC₅₀ or PRNT₅₀
✔️ Graphical representation of neutralization curves
✔️ Detailed assay report with interpretation